QX200 Droplet Generator

Description

Use the QX200 droplet generator to complete the first step of the Droplet Digital PCR (ddPCR) workflow by partitioning ddPCR reaction mix into 20,000 nanoliter-sized droplets for up to 8 samples per run. The droplet generator is used with the QX200 droplet reader for EvaGreen or probe-based digital PCR applications.

Associated components and consumables included: DG8 cartridges, DG8 gaskets, and cartridge holder

Required Instruments

The instruments below are not included with the QX200 system:

• QX200 Droplet Reader — reads positive and negative droplets using a two-color optical detection system in a serial manner
• PTC Tempo Deepwell Thermal Cycler (12015392) or C1000 Touch thermal cycler with 96–deep well reaction module (1851197)
• PX1 PCR plate sealer (1814000)

Required Accessories

• QX200 droplet generation oil for EvaGreen assays (1864005)
• Droplet generation oil for probes (1863005)
• Droplet reader oil (1863004)

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

• QX200 ddPCR EvaGreen supermix (1864034)
• ddPCR supermix for probes (1863010)
• Droplet PCR supermix (1863024)
• One-step RT-ddPCR kit for probes (1863022)